Roche LightCycler 480 || Real Time PCR Benchtop System

Description

Roche LightCycler 480 || Real Time PCR Benchtop System

Includes: One Roche Light Cycler and Accessories.

The LightCycler® 480 System is a proven high-performance, medium-to high-throughput PCR platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Benefit from cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity. Generate meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines, and analyze true raw data without the need for passive reference dyes or normalization plates.

The ideal combination of speed, accuracy and versatility

The LightCycler® 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as an automated high-throughput solution.

Additional information

Weight	121 lbs
Dimensions	21 × 20 × 23 in
Manufacturer	Roche Diagnostics

Features/Benefits

Features:

Rely on the LightCycler® 480 System to perform:

Gene Quantification

Gene detection

Absolute quantification

Relative quantification/gene expression analysis

Genetic Variation Analysis

Melt-curve genotyping with HybProbe- or SimpleProbe probes

Endpoint genotyping with hydrolysis probes

Gene Scanning or Methylation analysis with High Resolution Melting (HRM)

Multiple Plate Analysis

96-or 384-well plates

Fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis

Basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves

Wide range of probe formats and fluorescent dyes

White or clear multiwell plates to best fit your assay format, application, and workflow

Specifications

Dimensions	W x D x H: 24 in x 24 in x 21.5 in (57.4 cm x 58.8 cm x 49.7 cm)
Weight	121 lb (55.6 kg)
Power consumption	200–240 Vac (50/60 Hz, 1500 VA)
Reaction volumes	5 μL–20 μL (384-well) 10 μL–100 μL (96-well)
Temperature control	Peltier-based heating/cooling from 37°C–95°C (20° starting temperature to perform specific melting curve analyses)
Heating rate	96-well block: 4.4°C/s 384-well block: 4.8°C/s
Cooling rate	96-well block: 2.2°C/s 384-well block: 2.5°C/s
Excitation	LightCycler® 480 LED Lamp (430-630 nm)
Detector	Cooled monochrome CCD camera
Filters	Excitation (nm): 440, 465, 498, 533, 618 Detection (nm): 488, 510, 580, 610, 640, 660
Computer	Pentium PC with Windows 7

Test Menu

Gastro Parasite Panel	Gastro Virus Panel	Gastro Bacteria Panel	Respiratory Influenza Panel

Giardia2	Novovirus GG12	Aeromonas2	Influenza A2

Dientamoeba fragilis2	Novovirus GG22	Yersinia enterocolitica2	Influenza B2

Cryptosporidium spp2	Sapovirus1,2	Campylobacter2	Influenza A H1 (H1N1)1,2

Blastocystis2	Rotavirus2	Shigella/EIEC2	Influenza A H31

Entamoeba histolitica2	Adenovirus1,2	Salmonella2	Influenza A H51

Emerging Pathogens Panel	Astrovirus1,2	Plesiomonas1	Influenza A H7 (H7N9)1

Zika Virus2	Enterovirus1		Influenza A H91

Respiratory Virus Panel	Respiratory Coronavirus Panel	Carbapenemase Panel	Respiratory Atypical pneumonia Panel

RSV1,2	Sarbecovirus SARS-CoV-22	KPC2	Pneumocystis jirovecii1

ParaInfluenza 11,2	SARS-CoV-2 (COVID 19) N-gene1	OXA-232	Mycoplasma pneumoniae1

ParaInfluenza 21,2	SARS-CoV-2 (COVID 19) E-gene1	GES1,2	Chlamydophila psittaci1

ParaInfluenza 31,2	SARS-CoV-2 (COVID 19) RdRP-gene1	VIM2	Chlamydia pneumoniae1

ParaInfluenza 41,2	MERS Coronavirus UpE1	NDM2	Legionella pneumophila1

Enterovirus1,2	MERS Coronavirus Orf1a1	OXA-482	Legionella spp/pn1

Bocavirus1,2	Coronavirus HKU11	IMP1,2

Parechovirus2	Coronavirus OC431	EHEC Panel	New Born Panel

Metapneumovirus1,2	Coronavirus 229E1	STX1-EHEC1	TREC1

Adenovirus1,2	Coronavirus NL631	STX2-EHEC1	KREC1

Picornavirus1,2	panCoronavirus1	EAE-EHEC1

Rhinovirus1	Bordetella pertussis1	Non-Culturable Panel	Filovirus Panel

	Bordetella parapertussis1	T. whipplei1	Ebola Zaire1

Tropical Panel	Bacterial Meningitis Panel	Resistance Panel

Zika1,2	Escherichia coli uidA1	MCR-12

Dengue1	Listeria monocytogenes1	Mycoplasma Macrolide1

Chikungunya1	Streptococcus pneumoniae1

Plasmodium genus1	Neisseria meningitidis1

	Streptococcus agalactiae1

	Haemophilus influenzae1